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Immunoblot signals were visualized by an ECL chemiluminescence substrate reagent kit (Pierce), and were quantified by densitometric scanning and image analysis using Quantity One software (Bio Rad Laboratories, Hercules, CA).Immunofluorescence LabelingMice were transcardially perfused with 0.1 PBS (pH7.4), followed by 4 paraformaldehyde in PBS under deep isoflurane anesthesia. Brains were postfixed for 24 in 4 paraformaldehyde in PBS at 4 and transferred to PBS. The sections were then washed three times in PBS and mounted with anti fading medium.

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