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Sequencing of Y chromosome single nucleotide polymorphisms (SNPs) on Skeleton 1 was carried out by on array DNA hybridization capture15 of 24 amplified Illumina16 sequencing libraries, using probes generated to cover SNPs relevant to the major European Y lineages, followed by sequencing on a single 100 SE Illumina Hiseq 2000 sequencing lane. This approach provided insufficient coverage for some SNPs and further typing was performed using targeted PCRs with the amplification products sequenced on an Ion Torrent PGM. Finally, we also generated a STR haplotype using the Promega PowerPlex Y23 system (see Supplementary Figs 3 and 4 and Supplementary Tables 3 5).
But, don’t agitate them, be calm and nice to them, and ask them why they are still present. I keep getting the view of my grandmother walking through my house, and for some reason, I don’t feel uncomfortable. Even when I’m home alone, I still don’t mind her.
Slide your arms through the bathing suit top, if you are a woman and have selected a one piece. Pull the straps over your shoulders and adjust the top to cover your breast area. If you are wearing a halter style bathing suit, you do not need to slide your arms through.
Ground an electrical outlet with no grounding wire by installing a Ground Fault Circuit Interrupter (GFCI) receptacle. The National Electric Code (NEC) allows three prong GFCI outlets to exclude ground wires due to the built in breaker switch that trips at the indication of a short. An electrician may insist on grounding a GFCI during installation, but it is not required for most applications.