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Sequencing of Y chromosome single nucleotide polymorphisms (SNPs) on Skeleton 1 was carried out by on array DNA hybridization capture15 of 24 amplified Illumina16 sequencing libraries, using probes generated to cover SNPs relevant to the major European Y lineages, followed by sequencing on a single 100 SE Illumina Hiseq 2000 sequencing lane. This approach provided insufficient coverage for some SNPs and further typing was performed using targeted PCRs with the amplification products sequenced on an Ion Torrent PGM. Finally, we also generated a STR haplotype using the Promega PowerPlex Y23 system (see Supplementary Figs 3 and 4 and Supplementary Tables 3 5).


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Ground an electrical outlet with no grounding wire by installing a Ground Fault Circuit Interrupter (GFCI) receptacle. The National Electric Code (NEC) allows three prong GFCI outlets to exclude ground wires due to the built in breaker switch that trips at the indication of a short. An electrician may insist on grounding a GFCI during installation, but it is not required for most applications.

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