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The central nervous system consists of the brain and spinal cord which controls, coordinates and regulates the body ability to maintain homeostasis while interacting with the environment. The central nervous system is housed and protected by the bones that make up the skull and 24 moveable bones called vertebrae that make up the spine. Between each of the 24 movable vertebrae of the spine emerges a nerve root that becomes what .
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A mouse GAPDH endogenous control was used as an active reference to normalize quantifitation of targets for differences in the amount of total RNA added to each reaction. TaqMan Universal PCR Master Mix (Applied Biosystems) was used for real time detection of cDNA. Thermal cycling condition comprised 2 at 50 10 at 95 40 polymerase chain reaction cycles, each consisting of 15 at 95 1 at 60 The data were acquired and analyzed with Sequence Detector 2.0 software (Applied Biosystems).